Moved: Calculating Compensation Manually (to BD FACSDiva. Mucking about in FlowJo is fun, especially when you can get immediate visual feedback from changing a transformation setting. The flowCore::biexponentialTransform function has the following call: biexponentialTransform (transformationId'defaultBiexponentialTransform', a 0.5, b 1, c 0. All other trademarks are the property of their respective owners. New: Adjusting Biexponential Scaling, Copying and Pasting Gates. FCS Express is a trademark of De Novo Software Inc. FlowJo is a copyright of the Trustees of Leland Stanford Jr. CytoFLEX and CytExpert are trademarks of Xitogen Technologies (Suzhou), Inc., a Beckman Coulter company. Beckman Coulter, the stylized logo, Kaluza are trademarks of Beckman Coulter, Inc. Unfortunately, for this version (9.7.7), FlowJo does not allow the user to set the maximum value to be viewed therefore, the decade display cannot be changed and stayed as a 7-decade plot.įor Research Use Only. In the “Edit Biexponential Transformation Settings” window, enter values that are higher than the default value then press enter, followed by selecting “Apply Now”. However, I find that in this version of FlowJo 8.7, all my colours must subscribe to the same settings under biexponential. Im using a 12 colour stain, and Ive used Define Biexponential Transformation in newer editions of FlowJo so that my cells are not sitting on the axis. Go to Platform – Biexponential Transformation – Manually specify TransformĢ. FlowJo on Mac - biexponential adjustment - posted in Flow Cytometry: Hi, Im using FlowJo 8.7 on Mac to analyze my cells. This view is now similar to the data as seen in the CytoFLEX CytExpert plot.ġ. When transporting CytExpert data files into FlowJo V9.7.7 the data appears as here: to address this challenge in FlowJo is called biexponential transformation. The new transformation appears in the plot: Biexponential scaling helps visualize data that is compressed against the. mac version and biexponential transforms There are two preferences and one user action involved in setting biexponential scaling in FJ Mac.
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